Regenerated of Broccoli (Brassica oleracea var. italica) Plants from Differentiation of the Hypocotyl Stems Callus of its Seedlings

Abstract

The current study produced broccoli plants (Brassica oleracea var. italica) from the differentiation the hypocotyl stems callus of its seedlings and succeed of their adaptation and transferred to the soil. The results showed the difference in ratio of callus initiation with different type of plant growth regulators Naphthalene acetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D), Indole-3-butyricacid (IBA), Benzyl adenine (BA) and their concentrations which used in this study. The interaction of IBA and BA had an efficient role of increasing the initiation ratios. Morishige and Skoog (MS) medium supplemented with 1.0 mg L-1 IBA and 2.0 mg L-2 BA superiority in registered initiation ratio reached 100% after 11 days of the cultivation, and with its continued growth it produced typical callus cultures. The continued of callus subculture on the same initiation medium led to spontaneous production of 142 shoots produced from 50 pieces of callus. These regenerated shoots easily rooted in the MSO medium with full strength at ratio 100%, and with continued growth and formation of efficient root groups, they were successfully adapted to the soil within the pots in the greenhouse.