Assessment of Biochemical Parameters and Study its Correlation in ß‑Thalassemia Major Patients and Healthy Controls in Kirkuk City, Iraq

Abstract

Background: Thalassemia is the name of a group of genetically (acquired) blood diseases, which include defects on the production ofhemeprotein, and fractional or complete damage to the combination of a particular sort of simple protein chain. The defect might have an effecton the “α, γ and δ” chains or might have an effect on few combination of the “β, γ and δ” chains within the same patient. Objective: The aim ofthe study was to evaluate the various protein parts in the ß‑thalassemia major (BTM) patients in the city of Kirkuk compared to the healthycontrol group. Materials and Methods: Seventy blood samples were divided into two groups – 45 patients with BTM samples; they visitedAzadi Hospital/Kirkuk city and 25 samples of healthy controls as a control group. Results: The results of the correlation coefficient showedthat there were significant positive correlation between total protein (TP) and albumin and albumin and albumin/globulin ratio and significantnegative correlation between globulin and albumin/globulin ratio. Finally, nonsignificant positive correlation between TP and thiol, TP andglobulin, TP and albumin‑globulin ratio, albumin/thiol, globulin and free amino and globulin and thiol and non‑significant negative correlationbetween TP and free amino, thiol and carbonyl, thiol and free amino, carbonyl and free amino, albumin and carbonyl, albumin and free amino,albumin and globulin, and globulin and carbonyl. Conclusion: The correlation studies between the above parameters indicated a negativerelationship between TP and free amine in β‑thalassemia patients. These results reinforce the importance of measuring both TP and freeamine concentration to investigate their development of β‑thalassemia complications. Also, measuring both thiol and free amine yields is alsoimportant for the same reason.