MUCO-ADHESIVE GEL OF LIPOSOMAL PROGESTERONE AND LIPOSOMAL PMSGVAGINAL FORMULA CHARACTERIZATION AND PREPARATION IN VITRO AND IN SITU INVAGINAL MUCOUS OF EWE

Abstract

Medroxyprogesterone acetate bearing Nano-liposomes in mucoadhesive geltablet (MAG Lipo P4)and Liposomal PMSG (Lipo PMSG)were prepared by the thin film method and evaluatedfor severalstandardization techniques and compared with Medroxyprogesteroneacetate sponges (MAPS) implicated the mucous of ewe attributes. The progestationalbioactivity of liposomal form and MAPS as compared with control was assessed by monitoring the effect on the formation of synchronization and superovulation;the liposomes encapsulate 75.41% of MAG Lipo P4 and 69.06% for Lipo PMSG. The liposomal progesterone was incorporated intoCarbopol-HPMCmucoadhesivegel and the hormone release in the vaginal strip was estimated and withdraws of degradable tablet determined in vitro~6. The Lamellarof the liposome in MAG Lipo P4 1-3 and Lipo PMSG 2-6 lamellae, Size of the liposome in MAG Lipo P492.19nm andLipo PMSG63.10nm, the osmotoleranceapproved set tolerated between 0.6-1% of NaCl concentration and pH challenge was stable between 6 to 8 of pH of the liposomal releasing hormone. The color of mucous share MAG Lipo P4 treated ewes were cloudier to less clear milkieras compared to MAPS treated ewes cloudyThe spinnbarkeit mucus score showed in MAG Lipo P4 treated ewe increase as compared with control groups and MAPS. The estrous cycle synchronization challenge showed Sodium, Potassium, Calcium, and Magnesium in vaginal mucus of MAG Lipo P4 were higher than other groups, Chloride and Magnesium were similar in MAPS and MAG Lipo P4 the Potassium in MAPS as higher than MAG Lipo P4 and both was lower than both controls groups. The protein concentration of mucus in MAG Lipo P4 ewes markedly increase as compared with MAPS treated ewes and both higher than control groupsMucus pH in both MAG Lipo P4 and MAPS was less acidic; nearby neutral, than positive control and negative controls.It can be concluded that MAG Lipo P4 formulation tablet containing progesterone exhibited potentialeffect by in vitroprogestational alteration of mucous content minerals and proteins and positive effect on spinnbarkeit. Moreover, an orchestrate regulation ofthe hormonal releaseand duration was achieved through the formation ofthe depot in vaginal mucosawith conducted minimalized dose and achieved better effects. However, detailed formulation use with promotion anew clinical dosage form less adverse reaction to establish the utility of synchronization and superovulation.