Effect of Zinc Nanoparticles in Leaves Content of Proline and Non-Enzymatic Antioxidants of Sunflower Helianthus annuus L. under Water Stress

Abstract

The experiment was carried out in the municipality nursery of Najaf Municipalities Directorate during the spring season 2018 to investigate the effect of zinc nanoparticles in the non-enzymatic antioxidants of sunflower plant under water stress and plant response to reduce the number of irrigation periods in the presence of zinc nanoparticles.The Randomized Complete Blocks Design was used in this experiment, which dealt with two factors and the results were compared according to the Duncan multiple test. The first factor in this experiment was the water stress factor (irrigation every 3 days, irrigation every 6 days and irrigation every 9 days) while the second factor represented the effect of using zinc (control , ZnSO4 and zinc nanoparticles (ZnO) in 100ppm,by spraying three times for both zinc( normal and nano).Water stress caused a significant increases in antioxidants such as proline, glutathione, flavonoids, anthocyanins and ascorbic acid. The results also showed that Zinc nanoparticles caused a significant increase in all studied traits, except the leaf content of ascorbic acid ASA, in which zinc in both types significantly decreased this trait.

Keywords

The experiment was carried out in the municipality nursery of Najaf Municipalities Directorate during the spring season 2018 to investigate the effect of zinc nanoparticles in the non-enzymatic antioxidants of sunflower plant under water stress and plant response to reduce the number of irrigation periods in the presence of zinc nanoparticles.The Randomized Complete Blocks Design was used in this experiment, which dealt with two factors and the results were compared according to the Duncan multiple test. The first factor in this experiment was the water stress factor, irrigation every 3 days, irrigation every 6 days and irrigation every 9 days while the second factor represented the effect of using zinc, control, ZnSO4 and zinc nanoparticles, ZnO in 100ppm, by spraying three times for both zinc, normal and nano.Water stress caused a significant increases in antioxidants such as proline, glutathione, flavonoids, anthocyanins and ascorbic acid. The results also showed that Zinc nanoparticles caused a significant increase in all studied traits, except the leaf content of ascorbic acid ASA, in which zinc in both types significantly decreased this trait.