STUDY THE N - ACETYLCYSTEINE EFFECTS ON GASTRECTOMY AND SOME BIOCHEMICAL PARAMETERS IN DOGS

Abstract

The project of research was carrying out to study the effect of N-acetylcysteine on the wounds which developed in the stomach after gastrotomy. Dogs were used in this study as experimental animals. Dogs were divided into two groups' treatment and control; each group contains 5 animals. The surgical incision was closed by two layers of sutures using absorbent sutures then closed the muscle and skin using traditional steps. The treatment group was treated using 3% of N-acetylcysteine fluid topically on site of operation, as well as N-acetylcysteine (140 mg/kg/ day) was administrated orally for 7 days. Gross examination and clinical signs were recorded after 7 days of operation. Blood samples were collected before the surgery and 7 days after the surgery for complete blood studies on blood samples in both groups. Additionally, tissue samples were taken from operation areas to study the histological change. Results of this study show that the healing of treated groups with N-acetylcysteine was disappeared after 3 days while in control groups the healing sings continued until 7 days after surgery. The mucous membranes in the group treated with N-acetylcysteine were healthier comparative to the control group after 7 days of operation. Openings of the surgical area in the control group suffered from adhesions led to hemorrhage during their separation while in the treated group there were no adhesions. Blood analysis and liver enzymes tests showed an absence of inflammation and regularity of all liver enzymes in the treated group. Finally, the treated groups showed the normal arrangement of tissue layers and no adhesion or increasing in collagen fibers.