Molecular Characterization Of MDR3 And MDR4 Genes Of Aspergillus Fumigatus Isolated From Lung Disease Patients

Abstract

Background:Triazoles are the mainstay of treatment for aspergillosis. However, azole resistance is an emerging problem reported worldwide in Aspergillus infection mainly caused by A.fumigatus. Increase azole resistance in A.fumigatus has reported with treatment failure and become a significant challenge in effective management of aspergillosis.Aim: The aim of this study is to investigate the triazole-resistant of A. fumigatus and underlying MDR pump genes in viable clinical isolates which obtained from patients suffering pulmonary infections in Thi-Qar province.Methods:The conventional Polymerase Chain Reaction (PCR) was used to confirm antifungal resistance by detecting the presence of MDR pump genes (MDR3 and MDR4).Results:The results of using this technique showed that A. fumigatusisolates were positive to MDR pump genes with 90% and 96% for MDR3 and MDR4 genes, respectively.Conclusions: Our study revealed that the MDR pump genes are predominant in azole resistance isolates. Furthermore, PCR was proven to be highly effective method for identifying these genes.