Study The Effect of Some Antibiotics on Some Species of Gram Positive Bacteria Causing Mastitis in Buffaloes in Baghdad Province

Abstract

This study includes collection of 100 random milk samples of buffaloes taken from different farms in Baghdad city from February 2009 to June 2009 by aseptic methods then Whiteside test is done on milk to identify the subclinical cases from the healthy ones while the clinical cases had been registered from farms by the help of clinical signs in addition to owners. There were (10) clinical cases and (12) subclinical cases. The milk samples were examined in laboratory by making bacteriological cultures on blood agar then the bacteria that found were identified by using morphological and microscopical characteristics depending on results of primary identification, biochemical and verifying tests then API test was used to confirm the type of isolated bacteria. The current study showed that Staphylococcus Spp. was the dominant bacteria upon the remaining Gram positive bacteria. (14) isolates of Staphylococci were isolated followed by( 6) isolates of Streptococci and then (2) isolates of Corynebacterium and (1) Gram negative bacilli. Antibiotic sensitivity test was done using (7) antibiotics which ordinarily used for treatment of mastitis in buffaloes and these antibiotics were Ciprofloxacin, Gentamicin, Penicillin, Tetracycline, Chloramphenicol, Erythromycin, and Streptomycin this study showed that the ciprofloxacin was the best antibiotics and 79.19 % of tested isolates were sensitive while the streptomycin was the least effective one on bacteria.