A Study on Actinorhodin-like Substance Production by Streptomyces IQ 45

Abstract

Production of pH-pigment (actinorhodin – like substance) was ascertained fromten Streptomyses isolates. Streptomyses IQ45 isolate was only isolated which produced pHsensitive pigment. The production of pH-sensitive pigment was detected by fuming overammonia. After extraction of this antibiotic, a number of physiocochemical characterizationswere carried out which involved (IR, UV, MP, CHN-analysis, and solubility test). Indicated thatthis antibiotic is an actinorhodin-like substance. TLC of the extracted substance showed a singlespot with Rf value equivalent to (0.26) which was close to that of actinorhodin.These antibiotics showed inhibitory activity against Staphylococcus aureus similar to that ofactinorhodin produced by Streptomyces coelicolor A3 (2). The productivity of this antibioticswas (45 mg/L) at pH 8.5 and (40 mg/L) at pH 7 from the mycelial mat and (10 mg/L) whenextracted from the liquid medium at pH7.