Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant


Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/orcarcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentallyrisk. Laccases (E.C. are phenoloxidases catalyze the oxidation of PAHs in thepresence of a mediator compound and hyacinth plant. In this study laccase from Trametesversicolor was immobilized into chitosan, and the potential to oxidize anthracene in thepresence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that theimmobilization enhanced the stability of laccase against temperature, pH, inhibitors and loadingtime compared with the other cases. The immobilized laccase-mediator system was as efficientas the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilizedlaccase–HBT showed a system oxidization more than immobilized laccase without (HBT) ofPAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with orwithout HBT. These results indicate a new chance for applying the immobilized laccase inbioremediation