Study of Biosynthesis, Characterization and Antibacterial Activity of Silver Nanoparticles against Pseudomonas Aeruginosa Isolated From Wounds from Some Baghdad Hospitals

Abstract

The aim of this research is to study the biosynthesis of silver nanoparticles using the aqueous extract of rosemary leaf (Rosmarinus officinalis) because it is cost-effective and contains many active biocompounds. Biosynthesized AgNPs were characterized using UV-Visible Scanning spectrophotometer to measure absorption peaks, λmax was 465nm. FTIR technology was used to detect effective groups in plant which are responsible for nanoparticles formation. Scanning electron microscope (SEM) used to investigate the shape and size of nanoparticles, the size of particles was 74.7nm. X-ray diffraction meter (XRD) was used for analysis of nanoparticle structures, particle qualitative analysis as well as particle size, the size measured was 24.3nm. Atomic force microscope (AFM) used for detection of dispersion and agglomeration of nanoparticles, in addition to size, shape, and composition, the average of size was 67.15nm. zeta potential measurement device was used to measure the stability of AgNPs, Surface zeta potential value was -8.01mV. Particle size analysis was used for the purpose of determining the size of AgNPs, the size of nanoparticles was 82.4nm. The effectiveness of silver nanoparticles was studied against Pseudomonas aeruginosa isolated from wounds.The results showed that AgNPs were effective against these bacteria.