Biosynthesis and characterization of silver nanoparticles fromfungal species and its antibacterial and anticancer effect


Silver nanoparticles have gained considerable importance in recent years due to their diverse medicinal activities. In the presentstudy, we have explored filamentous fungiPenicillium italicumfor the extracellular biosynthesis of silver nanoparticles (AgNPs)and evaluated its antibacterial and anticancer effects. The nanoparticles were characterized by using UVeVisible and Fouriertransform infra-red (FTIR) spectroscopy and transmission electron microscopy (TEM) analysis. UVeVisible spectra showedspecific absorption peak at 422.67 nm which confirmed the presence of nanoparticles. FTIR spectroscopy analysis revealed thepresence of alcohols, phenols, alkenes, and amines that play major roles in stabilizing the synthesized AgNPs. Transmissionelectron microscopy (TEM) analysis showed spherical shape of AgNPs with size ranges from 14.5 nm to 23.3 nm. Antibacterialstudies againstStaphylococcus aureus,Salmonella enterica,Bacillus cereus, andEscherichia colithrough disc diffusion methodrevealed 20 mm (for 40mg/ml) of inhibition of zone especially forS. entericaand exhibited excellent synergistic effect whencombined with moxifloxacin and streptomycin. Further, in anticancer studies, these nanoparticles demonstrated good anticancereffect against HEp-2 cancer cell line with IC50 value at 30mg/ml through MTT assay.