EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING LEAVES EXTRACT OF OLIVE ON HISTOPATHOLOGICAL AND CYTOGENETIC EFFECTS IN ALBINO MICE

Abstract

This study was conducted to examine the possible effects of Ag-NPs synthesized using the olive leaf extract on histopathology and cytogenetic effct in mice. A total of thirty albino mice aged two months were divided into five treatment groups as follows: Group 1: served as control was administrated orally with 0.3 mg/kg bw of normal saline; Group 2 and 3 were administrated orally with varying doses of Ag-NPs synthesized on olive leaf extract (10 and 100 mg/kg bw, respectively) for 30 days; Group 4 and 5 were administrated orally with olive leaf crude extract (300 and 1000 mg/kg bw, respectively) for 30 days. At the end of experimental period, detection of possible chromosomal aberrations in blood samples and histopathology (liver, spleen, kidney, uterus and testes) were carried out. Statistically significant differences (P<0.05) were observed for the chromosomal aberrations in all Ag-NPs groups compared to control and to crude olive leaf extract groups. Histopathological study revealed various alterations in internal organs at high dose of Ag-NPs group including: inflammatory reaction, blood congestion, degeneration, fibrosis, mononuclear cells lesion and necrosis. Slight changes were identified at both doses of crude olive extract treated groups. Based on these results, oral administration of Ag-NPs could cause genotoxic and inflammatory responses in mice and this could be representing a risk to both environment and human health.