Evaluation of the Effect of Casein Phosphopeptide–Amorphous Calcium Phosphate Mousse versus Natural Raw Fresh Milk on Enamel Hardness After a pH Challenge

Abstract

Aims: The aim of this study was to evaluate the effect of CPP−ACP, raw fresh buffalo milk and cow milk on the microhardness of artificial initial caries lesions. Materials and Methods: 100 sound maxillary first premolars were collected and randomly divided into four groups, the teeth in all groups were subjected to pH cycling procedure then treated with: Group1: n (25) deionized water, group2: n (25) CPP−ACP tooth mousse, group3: n (25) fresh raw cow milk, group4: n (25) fresh raw buffalo milk. Enamel surface was assessed by Vickers microhardness test device at a baseline and after demineralization and after remineralization. Results: In all groups, there were high statistically significant reduction of surface microhardness after demineralization. There was high statistically significant increase in surface microhardness in all groups except deionized water after remineralization, the highest remineralization effect was found in CPP−ACP tooth mousse group followed by the milk groups that showed encouraging results with fresh raw buffalo milk having superior results then fresh raw cow milk. Conclusions: CPP−ACP tooth mousse, raw fresh buffalo milk and cow milk were effective remineralizing agents which were reflected by increasing the surface microhardness of artificial initial caries lesion, but with different abilities, CPP−ACP tooth mousse was the best followed by buffalo milk then cow milk.