Effect of pomegranate peel extract and Thyme leaves extract on growth of Escherichia coli isolated from infant diarrhea


This study were done in Al Batoul Teaching Hospital Baquba, Diyala Province, Iraq from 25/ 9/2016 to 1/2/2017, two hundred Stool samples were collected in sterile container from infants suffered from diarrhea with the age less than two year. All stool samples were cultured on MacConkey agar to isolation and identify Enteropathogenic Escherichia coli and then sub cultured of expected isolates on the Eosin Methylene Blue agar medium (EMB) , identification of bacteria on the base of morphological characteristics ( colony , shape , Gram stain ), and biochemical tests were done according to standard procedures, then confirmation of diagnoses were performed by by Api20E and VITEK 2. Sensitivity test of Escherichia coli isolates to fifteen antibiotics were performed to select the multi drug resistance isolates for investigation the inhibition effect of pomegranate peel extract and Thyme leaf extract on those isolates ,the results showed a high antibacterial effect of alcoholic extracts of pomegranate peel followed by the hot and cold water extract, were the rate of diameters of the inhibition zone at the concentration 100 mg / ml 21.92 , 18.02 and 14.02 mm respectively. The inhibitory activity of Thyme leaf extract at concentration of 100 mg /ml for alcoholic, aquatic extract hot and cold water extract were 11.3,9.8 and 3.22 mm respectively. We concluded that both alcoholic extracts of pomegranate peel and Thyme have a good inhibitory effect against growth of multi drugs resistance pathogenic Escherichia coli .