Isolation and identification of bacteria from computers /laptops used in the internet cafes in Al-Najaf province.

Abstract

Background: The ability of a computer to act as fomites has been previously documented in healthcare[21]. This study performed in Al-Najaf province on 150 computers to illustrate the role of the computers/laptops in the dissemination of the pathogenic bacteria. Method: Normal saline moistened swabs used to collect the samples from the computer keyboards, mice and power supplies. These swabs routinely transported and cultivated on the different culture media, followed by routine identification, antimicrobial susceptibility patterns performed by using Bauer and Kirby standard method (1966). Results: The results reveal that the predominant bacterium was the genus Bacillus cereus (47 %) among other Gram positive bacterial genera, followed by Staphylococcus aureus (31.4 %), Streptococcus spp(16.7%) and non-typeable bacteria (4.9%) respectively, while the bacterium E. coli was the prevalent genus among other Gram negative genera (65.31 %), while the bacterium Klebsiella pneumoniae recorded (34.69 %) in all computer keyboards, mice and power supply bottoms. On the other hand the antimicrobial susceptibility patterns expose that the most effective antimicrobial agents was Trimethoprim- sulfamethoxazole against Gram positive bacteria, and Amikacin against Gram negative bacteria.