Immunogenic Diagnosis of Human Cytomegalovirus Infection in Aborted and Pregnant Women in Babylon Governorate

Abstract

"Cytomegalovirus (CMV) infection remains the most common potentially serious viral complication in humans and is a major cause of congenital anomalies in women". "Subjects of this study were investigated for the role of human cytomegalovirus (HCMV) in Abortion". This study was designed to search for some causative and risk factors in 70 aborted women who had history of spontaneous recurrent abortion as case group compared with 20 healthy pregnant women as control, their age ranged between (15-45) years. Enzyme-linked immunosorbent assay (ELISA) was performed to detect anti Cytomegalovirus IgG, IgM antibodies and molecular study using PCR technique to confirm the immune detection".The results show that among 90 samples were evaluated for CMV infection, 62(89%) were sera positivity toward anti-(Cytomegalovirus) IgG antibodies, while the 65 (93%) of patients were negative for IgM antibodies. "While the results obtained from the control were 9 (45%) subjects positive for IgG and 55 % were negative for IgG and all of them were negative for IgM". The finding showed that furthermost of Cytomegalovirus infections among females through ages ranging among 20-29 years. "The outcomes of molecular detection by PCR amplification for presence of HCMV DNA in serum samples revealed that HCMV genome showed that small number of samples 13 (19%) were HCMV DNA detectable in aborted women while less than pregnancy women" 3 (15 %).