Spectrophotometric Determination of Salbutamol by Oxidative Coupling Reaction with 1-Naphthylamine-4-Sulfonic Acid in the Presence of Potassium per Sulfate

Abstract

Background: Oxidative coupling reactions are among the most important reactions that have wide applications in chemistry, and theoxidative coupling process involves the interaction of two or more organic substances in the presence of an oxidizing agent, where anoxidation process occurs for these substances forming intermediate compounds that interact with each other to form a colored productthat can be determined by either spectrophotometric, polarographic, or various chromatographic methods and these reactions wereused to quantify many different organic and inorganic compounds. In this article, the spectrophotometric determination of salbutamolwas done by an oxidative coupling reaction with the reagent 1-Naphthylamine-4-sulfonic acid in the presence of potassium per sulfate.Objective: In this study, we developed a simple, rapid, sensitive, selective, and accurate UV-visible spectrophotometric method foranalyzing salbutamol in pure drugs and pharmaceutical formulations. Materials and Methods: Based on oxidative reaction with1-Naphthylamine-4-sulfonic acid at pH 11.24, form a yellow-orange colored product in alkaline medium has a maximum absorbanceat 470 nm. The Beer’s law is followed in the concentration range 5–23 μg/mL. Results: The product was stable for 60 min, molarabsorptivity was found to be 0.8054 × 104 L·mol−1·cm−1, and sandal index was 0.0296 μg·cm−2 with a relative error (RE) of 0 to −0.79,and a relative standard deviation (RSD) of 0.54%–1.84%. Detection limit (DL) was 0.227 μg/mL, quantitative limit (QL) was 0.756μg/mL, correlation coefficient was 0.9998, and recovery was 99.51%, respectively. Conclusion: The method was found to be accurate andprecise, as indicated by recovery studies which is up to 100% and relative standard deviation is not more than 2 that is good result. Theproposed method has been successfully applied in the determination of preparations containing salbutamol.