Effect of blood storage on certain hematological parameters

Abstract

When blood was stored outside the body some hematological and biochemical changes will takes place resulting in reduced red blood cells survival which is important drawback when transfused into the circulation of a recipient. This study was done to determine certain hematochemical effects on blood when stored during different periods of time (at 7 storage periods (from zero time up to 35 days) in both sexes using CPDA1 solution as preservative. Fifty blood donors (25 males and 25 females) who were attending the Central Blood Bank, Al-Zahrawi Hospital, Mosul (IRAQ) during the period from 1st October 2002 to 31st March 2003. A blood sample consisted of 50 ml was taken from each blood bag and this was divided into 7 portions, each contained about 7 ml of blood added into plain tubes. Blood in one of these tubes was analyzed immediately. The other six tubes were analyzed later on at intervals of 3 days, 1, 2, 3, 4, and 5 weeks. The blood samples were analyzed for hemoglobin (Hb), plasma (Hb), packed cell volume percentage (PCV %), methemoglobin and sulfhemoglobin. The results of this study showed that there was a significant decrease (P<0.05) in Hb, packed cell volume, methemoglobin and sulfhemoglobin while there was a significant increase (P<0.05) in plasma Hb. Moreover, when the Hematochemical parameters of donor blood samples of males is compared with that of female donors' blood sample at the same periods of storage no significant differences were noted between them. The possible effects of stored blood that it may undergoes unavoidable hematochemical changes that lead to decrease active desirable substances such as hemoglobin and viable red blood cells. So blood transfusion is preferable during less than 7 days of storage Moreover the Hematochemical parameters of donor blood samples of males was compared with that of female donors blood sample at the same periods of storage and no significant differences was noted between them