Microbiological Studies on Bacteria Associated with Several Oral Disease And Dental Caries

Abstract

A total of (80) swabs were collected from mouth of patients suffering from dental and oral disease of both sexes and their ages ranged from 1-60 years which are ( referred) to consult the dental clinics and specialized centers of dental in AL-Hawija city from 2009.12.1 to 2010.4.30 ,In order to isolated and identified the causative agents that associated with oral disease and its antibiotics sensitivity against some antibiotics in addition to study the some factors that a effected and aided the occurrence and prevelance of these agents in mouth. Gram positive bacteria were ahigh number especially Streptococci – viridans which get of (4) species , form it the more important it’s the Streptococcus mutans (20) isolats and in rate 25% , then S. sanguis (14) isolates 17.5% , S.mitis ( 13 isolates ) 16.3% and S.salivarius (12 isolates)15% .For the Staphylococci were isolated Staphylococcus epidermidis (8 isolates) rate 10%,for the Lactobacillus ,two species were isolated (6 isolates ) in rate 7.5% for L. acidophilus, the other species L. casei (5 isolates) 6.2% , the most other Gram positive bacteria were rare in isolation and at defferent(2 isolates ) rates 2.5% Actinomyces viscosus.The ability of some bacteria to tolerate different salt concentrations & PH level . It was found that Staph. epidermidis was capable of toleration of the highest salt concentrations( 10%) while L. acidophilus was sensitive for all of the utilized concentrations, but it showed its high capability to grow on acidic medium (PH=4) more than other bacteria . The antibiotic sensitivity test for isolates against (9) antibiotics showed that the Cefotaxim was the best antibiotics and have higher effect on bacterial isolates ,then Ciprofloxacin and Amikacin .While the antibiotics Trimethoprim, Ampicillin and Amoxicillin were less effect on these isolates.