Polarized Light Microscopical Features of Erosion and Remineralization Process by Two Different Mouthwashes

Abstract

Aims: The study aimed to estimate the in vitro effect of a cocoa bean husk extract (CBHE) mouth rinse in comparison with that of a fluoridated mouth rinse on microscopic features of enamel surface after subjecting the teeth to two types of energy drink challenge. Materials and Methods: Twenty sound maxillary first premolars were collected and randomly divided into four groups, the teeth in the first two groups were immersed in the tiger energy drink for 14 days, then the teeth in the first group were exposed to a CBHE mouthwash and the second group was exposed to a fluoride mouth wash for 7 days. While the remaining teeth in the second two groups were immersed in the red bull energy drink for 14 days, then one group in a CBHE mouth wash and the other in a fluoride mouth wash for 7 days. Enamel surface was evaluated by a polarized light microscope at a baseline, after erosive challenge and after remineralization with the different treatment materials. Results: Microscopic images confirmed that prolonged subjection of dental sample to energy drink could crack or erode the tooth surface and between the two energy drinks, tiger energy drink had a somewhat more destructive microscopic feature. Besides that, both mouthwashes were efficient in re-mineralizing the defected area, and CBHE mouth wash revealed somewhat a high amount of remineralization which appeared in the polarized light microscope images being more dark areas in comparison with fluoride groups . Conclusion: Within the limits of the current study, CBHE and fluoride mouth rinses had an effective re-mineralizing ability after erosive energy drinks which had a destructive effect on tooth enamel. CBHE mouth wash showed somewhat a high ability of remineralization when compared with fluoride groups