Antibacterial Activity Evaluations of Thymus vulgaris Essential Oil Extract Against Clinically Isolated Gram-Positive and Gram-Negative Pathogens


Thymus vulgaris commonly known as thyme, belongs to the family Lamiaceae which is flowering plant native to Southern Europe, and world widely distributed. Thyme is popular plant that grow as wild plant in the mountains of north Iraq and it has been used for centuries traditionally as herbal tea, flavoring agents, home remedy, drug and perfume. This study investigates the antibacterial effect of thyme essential oil extract. Thymus vulgaris collected from Piramagron Mountain 50 km far from Sulaimani city-Iraq. The sample was identified by comparison with a specimen present at the herbarium in College of Agriculture/ University of Sulaimani then the leaves separated, dried, powdered and its essential oil extracted by hydro distillation method. The essential oil of thyme used as antibacterial and tested against five distinct clinically isolated bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus xylosus, and Staphylococcus epidermidis) in Microbiology lab, at Komar University by Agar well-diffusion method and the results showed strong inhibitory activity of the oil extract that killed both gram-negative and gram-positive bacteria used in this study with a different diameter of inhibition zone (mm). The data of this study suggests that Thymus vulgaris essential oil has potential applications as antibacterial agent against different species of pathogenic bacteria and can be used as a safe plant source for discovering new antibacterial agent.