Synthesis, Characterization of 2-(5-Chloro 3,3-dimethyl- 1,3-dihydro-indol-2-ylidene)-3-(4-methoxy-phenylimino)-propionaldehyde and Testing its Cytotoxic Activity Against Cancer Cell Line


Anew compound of indoleSchiff base derivatives have been synthesized by reaction of 2-(5-Chloro-3,3-dimethyl-1,3-dihydro-indol-2-ylidene)-malonaldehyde with 4-methoxy aniline. The chemical structure of the synthesized compound was characterized by TLC, FT-IR, 1H, 13C NMR and APT 13C NMR. The in vitro anticancer activity of the newly synthesized compound tested against AMJ13 breast cancer cell line. The revealed data showed that compound have promising anticancer activity. AMJ13 cell line was time dependent in both 40 and 60 μg/ml and the ideal inhibition rate to AMJ13 cells growth is 66 and 68 % after 72hs. of exposure. However, the lower concentration 20 μg/ml also displayed cytotoxicity against the tested cell line with 50 and 68 % inhibition rate determined after 48 and 72hs. of exposure. The Concentrations 10 and 20 μg/ml gave less than 10% inhibition rate when tested against REF cell line viability for 24, 48 and 72hs. Schiff bases compoundssuggested to have a potential effect by inhibitingcancer cell line, and further studies needed to determine the mechanism of their antitumor activity.