PREPARATION OF C-REACTIVE PROTEIN LATEX REAGENT OF HUMAN SERUM

Abstract

ABSTRACTC-reactive protein (CRP) is an acute phase reactant the concentration ofwhich increases significantly following tissue injury or inflammation. It ispresent in the albumin fraction of the serum of patients and is antigenic uponinjection into rabbits. We report here the development of a local latex reagentcoated with rabbit anti-CRP. For this purpose CRP was isolated from pooledsera of patients and injected into rabbits. CRP rabbit antiserum was purified byusing ammonium sulfate precipitation. Followed by passing on ion exchangechromatography (Diethylaminoethyl "DEAE" cellulose). Then, latex particleswere coated with purified antisera. The prepared CRP latex reagentagglutination was observed upon the addition of patients' sera and comparedwith that of normal sera. In addition, it was evaluated against a standard CRPlatex reagent. The prepared reagent gave positive results (100%) with patients'sera and the positive control of the standard kit. While, the negative control ofthe standard kit and 30% of the normal sera gave negative results.