Abstract

Agriculture and industrial waste are one of the most components that accumulate in the environment according to the activity of countries. Therefore, these wastes can be used as substrates to produce useful products like amylase enzyme as a friendly solution for this problem. The present research aimed to isolate a total of 100 bacterial isolates (50 from each soil type location) were primarily selected for the isolation of bacteria. Among them, the percentage of G. stearothermophilus bacteria was higher in Kirkuk soil than in Erbil soil, Bacterial growth was observed on cultured nutrient agar (14 that % 28) of positive bacteria were in Erbil government and (21 that % 42) of positive bacteria were in Kirkuk government. The ability of these isolates was tested for starch degradation using a solid starch medium at 55 °C for 2 days. Results indicated that only thirty-five isolates were selected for their capability to produce amylase enzyme according to the diameter (mm) of clearing zones. Secondary screening of thirty-five isolates was achieved in a submerged liquid production medium with 1% soluble starch at pH (7) and 55° C for 2. All isolated bacteria were identified according to morphological, and biochemical characteristics and by VITEK® 2 Compact BCL kit. Four agriculture wastes (wheat bran, broken rice, banana peel and potato peel) were used as a substrate for amylase production, the most active isolates AB1, AB43 and AB97 were inoculated in a submerged liquid production medium with 1% of each waste at 55˚C for 2 days, then quantitative of amylase was determined, which shows that potato peel had a greater result among all other substrate and all food waste had a greater result than positive control which was Nutrient broth with adding %1 soluble starch.

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The article was added to IASJ on 2022-12-03

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