Biomimetic Enamel Remineralization: A Comparative in vitro Study of Self-Assembling Peptide, Fluoride Varnish, and their Combination at Different Times by Surface Microhardness Test


Aims: This study was aimed to evaluate the efficacy of self-assembling peptide, fluoride varnish, and their combination to remineralize the artificial carious lesion in vitro. Materials and methods: Sixty extracted sound premolars were randomly divided into four groups. The artificial carious lesion was created by immersion the specimens in the demineralizing solution. The enamel surface treated with: Group1: (n=15) artificial saliva alone, group2: (n=15) fluoride varnish, group3: (n=15) self-assembling peptide, group4: (n=15) selfassembling peptide + fluoride varnish. The enamel surface was assessed by Vickers surface microhardness at the baseline, after demineralization, 1-week, and 4-weeks after remineralization. Results: all four groups exhibited high statistically significant differences between the different times of test periods, In 1 and 4-weeks after remineralization, the greatest surface microhardness mean value was seen in the self-assembling peptide + fluoride varnish group followed by self-assembling peptide group then fluoride varnish group while the artificial saliva group showed the least surface microhardness mean value with a highly statistically significant difference between them. Conclusions: self-assembling peptide superior to fluoride varnish in remineralization ability and this potential is boosted when they combined together. Relating to surface microhardness, remineralization is a dynamic process that increases with time