The role of Lactic acid bacteria in combination with conventional antibiotics against bacterial species isolated from genital tract of cattle


Numerous microorganisms are present in the cow's reproductive system from an early age. Other pathogenic microorganisms can occasionally enter and become inhabit cows' genital tracts, which can cause reproductive disorders that impair fertility. Vaginal samples were collected from cows in different fields of animal in Baquba city and from Veterinary clinic in Kanaan district during the period of October 2021to January 2022. Eighty one (81) local adult post calving were examined. The collected swabs or (loopfull) were submitted to culture by Streaking on to blood agar and MacConkey agar then incubated at thirty seven Celsius for 24-48 hr.From 81cows, all isolates were examined, the highest bacterial strain was isolated Staphylococcus spp. 75/268 (28%), followed by Pseudomonas spp. 61 (22.7%), E.coli 49 (18.3%), Sphingomonas spp. 46 (17.1%), Kocurea spp. 23 (8.6%), and Granulicatella spp. 14 (5.2%). Lactobacillus planrerum had positive effect and caused bacterial growth inhibition of for Pseudomonas spp., Kocurea spp. and granulicatella spp. While, Lactobacillus acidophilus had positive effect and caused inhibition in bacteria growth of Staphylococcus spp., pseudomonas spp., kocurea spp., and Granulicatella spp. When CFS of LAB was combined with different types of conventional antibiotic discs had growth inhibition effects against many types of bacterial isolates. Key