Turmeric extract ointment and diode laser for treatment of open wound

Abstract

Objective: The study was aim to evaluate the effects of topical application of 20% turmeric extract ointment and 8J/cm2, 660nm λ diode laser therapy in treatment of open wounds in mice model. Methods: Thirty adult male mice, approximately weighing (50g), and aged (60-70 days) were utilized and randomly divided into two equal groups. Mice were anesthetized by IM injection mixture of Ketamine and Xylazine (80 mg/kg BW of Ketamine and 10 mg/kg BW of Xylazine). Animals were prepared for aseptic surgery, and then made two parallel vertical circular full thickness (0.5 cm) skin incisions on the back of each animal. G1 (control group) left without treatment. G2 (turmeric extract ointment + diode laser treatment group), in which the cranial wounds were treated with a topical application of 200 mg / cm² of 20% turmeric extract ointment in addition to 8J/cm2, 660nm λ diode laser treatment once daily for 7 successive days post wounding (PW), and the caudal wound was left without treatment as a control in the same animal. Wounds were evaluated both grossly and histopathological. For histopathological assessment, specimens were taken after 7, 14 and 21 days PW. Results: Grossly at 7, and 14 days, the size of the treated wounds in the treated group were significantly P < 0.05 smaller than that of the control group and control wounds of the same animal, whereas the readings converged at 21 days. Microscopically; G1 on day 7 exhibited thin early reepithelialization of epidermal layers, the dermis had developed new blood vessels, infiltration of inflammatory cells, and the dermo-epidermal interface had vacuolations. At 14 days, the epidermis developed stratum corneum hyperkeratosis, thin irregularly arranged re-epithelialized layers. Acantholysis, inflammatory cells, blood vessels, accumulation of fibroblasts and thick evenly collagen fibers in the dermis. G2 at 7 days PW displayed hyper cellular well-regenerated epithelial layers, and accumulation of collagen fibers, inflammatory cells, and fibroblasts in the dermis. At 14 days PW, thick well-differentiated epidermal layers resting on clear basement membrane were seen. In the dermis there were dense mature collagen fibers, numerous hair follicles and sebaceous glands. At 21 days PW, the regenerated epidermis was laying on the foundation membrane, giving it its typical look. The dermis layer also had a large number of hair follicles that are at various stages of development along with collagen fibers, and fibroblasts. Conclusions: Used of 20% turmeric extract ointment with diode laser had a synergistic effect on the healing of open skin wounds.