Turmeric extract ointment for treatment of open wound

Abstract

Objective: The study was designed to investigate the effects of topical application of turmeric extract ointment in treatment of open wounds. Methods: Thirty male adult mice weighing approximately (50 gm), and aged between (60-70 days) were utilized and randomly divided into two equal groups (n15), G1(control), and G2 (turmeric) groups. Two open parallel (vertical or horizontal) circular full-thickness (0.5 cm in diameter) skin incision on the back of the animals were made by using punch machine. One incision was treated and the other was left as control in the same animal. Wounds of G1 were left without treatment. Left wounds of G2 were treated with a topical application of 20% turmeric extract ointment, in a dose of 200mg/cm2 once a day for 7 successive days. Assessment of the wound healing was done grossly and microscopically. Specimens were taken for the two groups after 7, 14 and 21 days PW for histopathological evaluation of the healing process. Results: Macroscopic results were shown significant P < 0.05 decreased of the size of wound in treated group in compare with control group and control wounds of the same animal at 7, and 14 days, while the readings become near each other at 21 days. Microscopic results of G1 show, thin early re-epithelialization of epidermal layers, hyperkeratosis, and presence of acantholysis due to inflammation. In the dermis there was newly formed blood vessels and infiltration of inflammatory cells, and vacuolization at the dermoepidermal junction at 7 day. At 14 days there was thin irregularly arranged re-epithelialized epidermis layers, with hyperkeratosis of stratum corneum, and a gap or acantholysis between epidermis and dermis later. The infiltrations of inflammatory cells and congested blood vessels were evident in the dermis. Also there was accumulation of the fibroblasts, and evenly arranged of thick collagen fibers in the dermis beneath the wound site. Microscopically G2;at 7 day PW, exhibit completely regenerated thick epidermis, severe infiltration of PMNCs and numerous multi-oriented new blood vessels, along with additional new collagen fibers. At 14 days PW well thickening of epidermis were seen upon deep area of granulation tissue. The field showed less cellular and more collagen, palely of hair follicles, swollen sebaceous glands, and the duct of sweat glands can be notified. Whereas at 21 days PW, well organized four layers of epidermis including a thick layer of keratin were seen in the skin wounds. The superficial dermis (SD) and the deep dermis (DD) are less cellular, contain more mature collagen fibers, hair follicles, sebaceous glands, and sweat gland. Conclusions: The treatments of open skin wounds with turmeric extract ointment 20%, were seen improve the healing process and accelerate the proliferation, wound contraction, maturation and remodeling phases of wound healing.