Receptors ( TLR2 , TLR4 and TLR9 } in gene bank – NCBI for patients with tuberculosis in Basrah – IRAQ
Abstract
A case-control study has been carried out among patients with Tuberculosis,who attended to the Consultation Center of Chest and Respiratory diseases of Basrah province during1st September 2020 to 1st June 2021. From total number of (176) patients with Tuberculosis (TB) were taken from Basrah province that included in the present study. The molecular study include extract of DNA from blood of TB patients and control group by using specific primers in conventional PCR, the amplification of all extracted DNA from blood samples was preform and confirm by using electrophoresis with (70 volt / 60 minutes) on agarose gel (1%), the results of gene expression revealed that the amplified DNA(PCR product) was 154bp for TLR2 with presence ratio 85.2% from total samples, 143bp for TLR4 with a presence of 100.0%, and 177 bp for TLR9 with a presence ratio 98.8%.In the sequencing study the results from sequencing of TLR2,TLR4 and TLR9 were showed as follows: there were clear convergence between our TLR2 gene and that of the GenBank database (NCBI) with identity 109/114 (96%) in forward-TLR2 had five mutations appeared as G to A; T to A; --- to A; T to C and T to C. In addition, the reverse-TLR2 was shown an identity 106/108 (98%) in comparison with GenBank database (NCBI) that founded two mutations in reverse TLR2 as T to C and C to T. And there was a low convergence between our TLR4 genes and that of the GenBank database (NCBI) with identity 111/113 (98%) in forward-TLR4 that were recorded two mutations appeared as G to A and C to ---. In the other side the reverse TLR4 shown an identity 106/113 (94%) when compared with GenBank database (NCBI) seven mutations were reported as T to ---; T to ---; T to G; A to C; A to G; A to C and T to C. And there was a highly convergence between our TLR9 genes and that of the GenBank database (NCBI) in identity 132/134 (99%) that founded two mutations in the forward-TLR9 as --- to C and G to C. And for reverse-TLR9 showed no mutations when we compared with GenBank (NCBI) database shown identity 128/128 (100%), there are no studies interested with sequencing of TLRs with relation of tuberculosis so we will compare our results with studies on other diseases
Keywords
tuberculosis, Basrah, Toll- Like Receptors, TLR, gene, DNA sequence, phylogenetic trre, gene bank, NCBIMetrics