Effect Alkaloid and aqueous extraction of Convolvulus Scammonia on microtubules of CHO cell line(china hamster)

Abstract

The study evaluated the effect of selected on crude Alkaloid and aqueous extraction fromroots of ( Convolvulus Scammonia) the microtubule network of CHO cell line (chinahamster) GFP tubulin labeled and the recovery of its disruption. Model experiments wereused for demonstration of the quantification of microtubule network changes induced bycrude alkaloids using computer-assisted image analysis.Cells were treated with Alkaloid and aqueous extraction from roots of (ConvolvulusScammonia) at various concentrations from 2 μg/l to 800 μg/l for 60 min, or with crudealkaloid at a concentration of 4615 μg/l and 9230 μg/l for 60 min. Microtubules weredetected by means of indirect Immunofluorescence.The damage was examined in a fluorescence microscope. Also, cells were treated for 60 minwith alkaloid at concentrations of 20 μg/l or 800 μg/l and the recovery process was studied intime intervals of 6, 7, 8, 9, 10 hours, or 8 and 12 hours, respectively. Differences in thearrangement of microtubules were assessed by means of quantification of the cytoskeletonchanges in cells treated with alkaloid at a concentration of 20 μg/l and in untreated controlcells.