GENOTYPING OF HLA-CLASS-II BY PCR-SSP OF IRAQI BREAST CANCER PATIENTS

Abstract

Background: Breast cancer incidence differs among women of different racial/ethnic groups. Several HLA alleles are associated with susceptibility or protection in Breast cancer, the particular allele varies depending on the racial groups.Objectives: This study was established to shed light on the possible association of HLA class II alleles with BC in Iraqi female patients.Subjects and Methods: The study included 60 subjects: 30 breast cancer patients, 12 patients with benign breast lesions as first control and 18 apparently healthy subjects as second control. Polymerase chain reaction-specific sequence primers (PCR- SSP) assay was conducted to assess HLA- typing.Results : A survey of the distribution of HLA-DR and HLA-DQ alleles frequencies yielded no evident of positive association between class II alleles and BC as compared with both control groups, but there was appreciable significant decrease in the frequency of DR*010101,0102,0201-0204,04-13 and DQB1*0401,02 alleles in BC patients as compared with healthy control (P=0.031).Conclusions: These findings demonstrated that HLA– DR*010101, 0102, 0201-0204, 04-13 and DQB1*0401, 02 alleles may confer protective effects against BC. Keywords: Breast cancer, HLA allele, PCR.