An Attempt to Utilize One Gel Double Staining Technique to Visualize DNA – Seminal Proteins Interactions


The main goal of this research is to test the rate of success of a low cost double staining method in visualizing any DNA-seminal proteins interactions. After collection of rabbit’s ejaculate and removing sperm cells, ion exchange chromatography was used to separate seminal proteins on the basis of their charge. Positively charged seminal proteins were eluted, lyophilized, and involved in this study. After incubation of this eluted group with the DNA, band shift assay was applied on this group. The results were compared. According to the results of this study, we demonstrated the necessity of utilizing a double staining technique for the same band shift gel in order to ensure weather real or false band substitutions were obtained.