CYTOTOXICITY EFFECT OF AQUEOUS EXTRACT OF BRASSICA RAPA ROOTS ON CANCER CELL LINES IN VITRO

Abstract

Effect of aqueous extract of Brassica rapa roots on three types of cancer cell lines; Hep-2, AMN-3 and Hela was studied in vitro.The results showed that, the cytotoxic effect of extract dependent on type of cells, amount of dose and exposure time.The concentration 1250 µg/ml gave higher growth inhibition , were ( 63 and 42 ) % to ANM-3 and Hep-2 respectively comparied with control 100% after 24 hours from exposure time , but inhibition gowth of Hela cells was 64% by 10000 µg/ml crud roots extract for 24 hours. Exposure time for 48 hours reduced the percentage of inhibited growth٫ only by 10000 µg/ml for extract; viability was 25 % for ANM-3 cells.Crud aqueous extract of Brassica rapa roots was have hormetic effect ( Hormesis ), because also induced the proliferation of cancer cells by used low concentration of extract after 72 hours exposure time.