Antibacterial activity of Thymus vulgaris and Prunus amygdalus extracts against bacterial wound infection

Abstract

Eighty clinical swabs were collected from Patients suffering from woundinfection, attending medical city hospital Sixty seven isolates were diagnosed ascausative agents, they were Klebsiella spp. (22.4 %), Escherichia coli (22.4%),Pseudomonas spp. (18%), Staphylolcoccus aureus(15%),Enterobacterspp(10.4%), Proteus spp (7.5%) Streptococcus spp (3%) and Acinetobacterspp.(1.5%); Eleven antibiotics were used for suscpitbility test of drugs. Most ofisolates were sensitive to lmipenem in percentage 97% and Amikacin inpercentage of 89.5% while most isolate were resistance for many antibiotics likeCefotaxim (89.5%), Tetracyclin (82%), Tobramycin (77.6%), Pipracilin (77.6%)and Gentamycin (77.6%), isolates from gram positive bacteria showedsensitivity (100%) for Vancomycin .Antimicrobial activity of Thymus vulgaris and prunus amygdalus againsthigher resistance isolates was estimated, using two folds dilution extracts in agardiffusion technique at concentrations of 1:2, 1:4 , 1:8, 1:16, 1:32 .Thymusvulgaris has shown anti bacterial activity against Acinetobacter spp., proteusspp. And Klebsiella spp.in all concentration were used .The lowest effect was onE.coli (12 mm, 8 mm) at concentration 1:2, 1:4. No effect was clear onPseudomonas spp., Staphylococus aureus and Streptococcus spp .Hexane extract of Sweet almond extract showed the best effect on Klebsiellaspp.,Enterobacter spp., Proteus spp. at all concentrations used, while E.coli andpseudomonas spp showed sensitivity concentrations of 1:2, 1:4, 1:8, the activityon Gram positive bacteria :Staphylococus aureus and Straptococus spp. was atconcentrations of 1:2 , 1:4 by measuring inhibition zone which was (8mm,7mm)and (10mm,9mm) respectively.