Identification of Mediterranean mutation in the G6PD gene on molecular level in Iraqi population


The study involved extraction of Deoxyribonucleic acid (DNA) from 71 samples of random G6PD patients and 85 samples of apparently healthy individuals from different Iraqi populations respectively, which was then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzyme to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely Mediterranean (Med). The results of the current study showed that Iraqies were affected by G6PD deficiency in a percentage of 7.2% and showed that the majority of affected cases were caused by Med mutation (95.8%). It could be concluded that Med mutation causes a serious impact on pediatric health and its the most prevalent cause of G6PD deficiency.