Assesment The Efficiency of Some Local Fungal isolates in The Production of Cellulase Enzyme

Abstract

Nine local strains of fungi were isolated and characterized they are related to the following genera: Aspergillus niger , Alternaria alternate, Penecillium sp, Rhizopus sp, Rhizoctonia solani, Fusarium solani, Drechslaria sp and Trichoderma viride , from the soil of University of Mosul .And another isolation Aureobasidium pullulans from orange leaves. The ability of isolated fungi to cellulase production in a solid media was investigated . Aquantitative test using liquid media is used to identify the most effective isolation for cellulase production. The isolation of T.viride gave highest production of cellulase (4.67) unit/ml . Carboxymethyl cellulose as a carbon source gave highest production of enzyme cellulase (4.72) unit/ml .The pH 5.5 considered the Optimum one for enzyme production, as it gave (4.96)unit/ml .