Immune response in BALB/c mice of polyamine oxidase isomer against infection with secondary hydatid disease III. Phagocytosis and delayed-type hypersensitivity


Polyamine oxidase activity in cerebrospinal fluid of normal children was determined, it was found that specific activity of crude PAO (64.16  8.18) enzyme unit/mg protein. Partial purification of the enzyme was performed by dialysis and ion exchange chromatography. Two main peaks of high PAO activities were obtained (I,II) with specific activity of (1201.92 and 1157.22) enzyme unit/mg protein, and with purification fold of 18.17 and 17.50 respectively.Immune response was studied to infection with secondary hydatid disease in BALB/c mice activated by partially purified CSF-PAO with spermine and infected with protoscoleces of Echinococcus granulosus. The pathological changes occurred in the mice activated by different concentrations of PAO (II) (200-1000 g) with constant concentration (200 g) Spm, were followed in comparison with positive and negative control group, along one month, depending on non-specific and specific immune response represented by phagocytosis and delayed-type hypersensitivity, respectively. Then pathological changes were followed in activated BALB/c mice with optimum concentration (800 g PAO(II) with 200 g Spm) in comparison with positive control group throughout two and three months, depending on the mentioned criteria.An increase in the non-specific (innate) and specific (cellular) immune responses, which represented by an increase in the rate of phagocytic index and foot pad thickness, respectively, in activated mice with PAO-Spm system throughout (1,2,3) months, in comparison with +ve control group.Therefore, it was concluded here that PAO (II) isolated from CSF with Spm could be considered as an effective immunomodulator against infection with secondary hydatid disease.