Effect of Exo - polysacchrides ( EPS ) isolated from Rhizobium leguminosarum bv. Phaseoli on nodules formation of Phaseolus vulgaris L. and callus initiation from Phaseolus vulgaris and Helianthus annus L. seedlings

Abstract

The aim of this study is to show the possibility of using Exopolysacchrides (EPS) which is isolated from Rhizobium leguminosarum bv. Phaseoli as a principle factor in nodules formation on the roots of Phaseolus vulgaris plants . In addition to use these compounds in callus induction from the hepocotyles explants of Phaseolus vulgaris seedlings and from hypocotyles , cotyledons and roots explants of Helianthus annus seedlings .The stimulation effect of EPS on the formation of nodules shaped structures was very clear since the percentage of these structures formation was 48% in nitrogen free medium (NF) supplemented with 0.2 mg/L (EPS) . In addition to increase the protein content of Phaseolus vulgaris plants wich grown in NF medium containing (0.05-0.1 ,0.2,0.4 ,and 0.8) mg /L EPS . The results showed the success of callus induction from different explants of Helianthus annus seedlings especially when it is used with Benzyl adenine (BA) , in agar solidified MS medium and the percentage of callus formation was 100% from hypocotyls and roots explants . Also the using of EPS alone in MS agar solidified medium helped callus induction and root formation from explants of Helianthus annus and Phaseolus vulgaris seedling.