Effect of Different Incubation Period and Different Carbon Sources on Production of β-Glucosidase Enzyme by local Isolate Fungus Trichoderma harzianum

Abstract

The ability of a local isolate fungus Trichoderma harzianum to produce the enzyme complex cellulase in a solid medium was investigated. It was observed that this fungus had intermediate activity to produce this enzyme and the activity ratio was 3.875. This isolate gave highest production of β-glucosidase on the basis of each ml of nutrient medium and each gram of biomass (3.366 unit/ml and 734.93 unit/gram) respectively after eight days of incubation. Sucrose as a carbon source gave highest production of β-glucosidase (3.316 unit/ml). Carboxymethyl cellulose gave highest production of β-glucosidase on the basis of biomass ( 13486.3 nit/gram).