Curing of some Antibiotic Resistance in Pseudomonas aeruginosa Isolates by the Action of Sodium Dodecyl Sulphate and Elevated Temperature


Twelve isolates of Pseudomonas aeruginosa were collected from different human infection cases, from Al-Razzi teaching hospital laboratories in Mosul. They were diagnosed using the API 20E System. The bacterial isolates were classified into four groups according to their resistance to six antibiotics (ampicillin, tetracycline, chloramphenicol, gentamycine, streptomycin and trimethoprim). No spontaneous curing for plasmid DNA content that confer resistance to above antibiotics, was observed in the tested isolates. But, by using the sodium dodecyl sulfate at concentration of 1% (W/V) with different incubation periods as curing agent, the bacterial colonies that lost their antibiotics resistance appeared with different curing rates. The results showed 100% of curing for streptomycin resistance while for chloramphenicol and tetracycline the curing percent occurred between 17% to 83% and little effect of SDS on ampicillin and trimethoprime resistance in some isolates. In addition, curing of plasmid DNA by elevated temperature (45°C) was carried out and the results revealed high percentage of curing for all antibiotics resistance as they were compared with that induced by the action of SDS. The percentages of bacterial colonies that lost their antibiotic resistance were more than 60% and reached to 100% for curing the ampicillin resistance in the three tested isolates.