Study Of ß-lactamases Producing Enterobacteria isolated from German cockroach (Blatella germanica) in hospitals


38 German cockroach (Blatella germanica) were captured to determine the potential role of the cockroaches in carrying ß-lactamases producing pathogens from hospital. Only cockroaches captured whole and live were utilized for the study. After that each cockroach was placed in a test tube with 5 ml sterile saline solution , and then homogenized. The resulting solution was cultured on the culture media. 30 isolates of Enterobacteria were identified , the most frequent were Klebsiella pneumoniae (40%), Pseudomonas aeruginosa (16.6%), E. coli and Proteus mirabilis( each 13.3%) , Serratia marcescens (10 %) and Enterobacter aerogenes ( 6.66%) . Bacterial isolates were tested against (10) antimicrobial agents: Amoxicillin, Erythromycin ,Cefoperazone , Cefazolin , Aztreoname , Ofloxacin , Cephalothin , Trimethoprim , Carbenicillin , and Gentamicin . Results showed that all the isolates were resistant to Erythromycin, Cefazolin and Amoxycillin , and all the isolates have showen multiple resistance for antibiotics. All isolates (100%) were susceptible to Aztreoname and Ofloxacin. The majority of isolates remained susceptible to Cefoperazone (70 %) and Trimethoprim ( 75 %) . The results showed also that 14 isolates ( 46.66 %) had the ability to produce ß -lactamase enzymes . 2 isolates ( 6.66 % ) were able to produce Extended-Spectrum ß -lactamases ( ESBLs).