أستخلاص وتنقية عديد السكريد الشحمي من جرثومة Citrobacter fruendii وتقدير وزنه الجزيئي ومحتواه الكربوهيدراتي والبروتيني

Abstract

Lipopolysaccharide (LPS) was extracted from local isolate of Citrobacter fruendii obtained from neonate suffering from diarrhea .Crude LPS was extracted from dried bacterial cells by enzyme digestion and hot phenol .The extract obtained from the water phase was partially purified by gel filtration , Molecular weight also was estimated , using standard proteins . It was about 260 KDa . Total carbohydrates of the crude and partially purified LPS were estimated , they were about (315 and 300 µg/ml ) respectively .It has been founded that the protein content of the crude LPS about (91.3 µg/ml ) and the purified one about (45.6 µg/ml) .