The Impact of Resistin and IL-6 On Type I Diabetes Mellitus (T1DM) and Its Duration in Children


ABSTRACT:BACKGROUND:Type I diabetes mellitus is characterized autoimmune destruction of β- cells . IL-6 is a cytokine (one of a class of immune system regulators ) it plays a role, in acute phase inflammatory response to cellular injury.Human Resistin gene is expressed in pancreatic islets , pre- adipocytes and bone marrow and is of relevance for inflammation processes.OBJECTIVE:To investigate serum concentration of resistin and pro inflammatory IL-6 in T1DMchildren and to study the impact of the duration of disease on these parameter. In order to shed some light on the mechanism of initiation and propagation of β-cell damage in those patients.SUBJECTS AND METHODS:Sixty diabetes children ( 33 males and 27 females), aged (1.3-13) years (mean± SD) (5.6±2.8) years , consecutively admitted to the AL-Mansour Teaching Hospital for children , were enrolled in this study. All had T1DM . Duration of disease varied from ( 0.13- 84) months . Age matching group of thirty healthy volunteer children , (18 females ,12 males) was included as a control without any family history of diabetes. Resistin and IL-6 were measured ( by enzyme linked immunosorbant assay , ELISA) .RESULTS :Fasting serum Resistin levels were lower in patients compared to controls although the correlation was not significant .However serum resistin levels were higher in females compared to males in both groups ( patients and controls) with a significant correlation between the groups of the same gender .Duration of the disease had no impact on either gender.On the other hand IL-6 showed a significantly higher serum level in patients than control.Un like Resistin , the duration of disease had a great impact on IL-6 serum levels as shown in text.CONCLUSION : Resistin levels in patients with (T I DM) non significantly lower than in control individuals , that’s meaning T I DM), as disease did effect the levels of serum Resistin sub clinically by the treatment with insulin leading to hyper insulinemia .Resistin antagonizes insulin action, leading to decrease in patients more than controls . Resistin levels tended to have higher in females than male ,however this trend did not reach statistical significant in total population due to sex hormone . The highly significant positive correlation between the levels of IL-6 and duration is due to persistente production and elevation for long time of patients with T1DM indicating of ongoing β- cell destruction .But Resistin was non dependent on the duration of the disease because human resistin gene is expressed in pancreatic islets cell.