A STUDY OF THE INHIBITORY EFFECT OF THE CAPAR, CAPPARIS SPINOSA L. AQUEOUS CRUDE LEAF EXTRACT ON THE HEP-2 AND HELA CANCER CELLS LINES

Abstract

Abstract This study was conducted to know the inhibitory effect of the crude aqueous Capar, Capparis spaniosa leaf plant on two cellular cancer lines human epidermoid larynx carcinoma Hep-2 and human cervix uteri epitheloid carcinoma Hela . Four leaf extract concentrations have been used (125, 250,500 and1000) μg/ml and the exposure time was (48 and 72) hrs .This study indicated a clear significant inhibitory effect (p>0.001) of the extract on the cancer lines ,Hep-2 and Hela ,even with the first low concentration .The cellular Hep-2 density was (0.340%) , whereas the density in Hela was (0.6545%) at the lowest concentration 125 μg / ml . The highest inhibitory effect of the extract was at (1000) μg/ml ,the cellular Hep-2 density (0.108%) and in Hela (0.266%) .The highest significant inhibitory effect of the extract (72) hrs of exposure time was (0.275%) Hep-2 and (0.4624%) Hela .The result of this study has indicated ahighest inhibitory activity of the aqucous leaf extract of Cpparis Spinosa on the density average of cancer cells for both Hep-2 and Hela .There was an inversely linear relation ship between exposure time and concentration on the growth of cells . As exposure time and concentrations have increased , the killed cells have increased also .

Keywords

:Cell line, Capparis