Duplex PCR primers for detection of Helicobacter pylori DNA directly from gastric biopsy samples


In order to avoid PCR inactivation because of nucleotides variability in primers annealing sites in template DNA molecules and confirmation the presence of medically important bacterium Helicobacter pylori in gastric biopsy samples with high accuracy, relatively low cost and easily, several primers pairs analyzed for duplexing capacity. One of 16s ribosomal RNA gene, and one of glmM (UreC) gene of this bacterium have duplexing capacity. Those primers are designed separately to detect the presences of H. pylori with high efficiency. Dimerization analysis resulted in allowable degree to use in duplex PCR, and the laboratory results confirmed applicability of this hypothesis. The present study concluded that the combination of these two primers is suitable for accurately detection of these bacteria directly from gastric biopsy specimens.Specialty: Molecular Microbiology, Computational biology