TOXICOLOGICAL AND SOME REPRODUCTIVE ASPECTS OF THE EFFECTS OF ALCOHOLIC EXTRACT OF COCONUT ON MALE ALBINO MICE

Abstract

In this study, two experiments had been done: Experiment (1), was designed to determine the acute toxicity of alcoholic extract of coconut ( Cocos nucifera ) fruit to the laboratory mice. Eight groups (6 mice each) of male albino mice were used in this experiment. The first group was drenched (1ml) physiological saline (as control group), while the other seven groups were given orally ascending doses (0.25, 0.5, 1, 1.5, 2, 2.5, 3 g/kg B.W.) respectively. After (24hr), all groups of mice were inspected for the presence of dead mice. The results of this experiment showed no mortality in all groups of mice and the extract is not toxic. Experiment (2), this study was designed to investigate the effect of alcoholic extract of coconut fruit on the efficacy of reproductive system of male mice. For this purpose, (24) mature male mice were allocated equally and randomly into three groups. Group (1) was given physiological saline and was considered as control group. Group (2) and group (3) were given 125mg/kgB.W./day and 200 mg/kg B.W. /day of alcoholic extract of coconut fruit respectively. All mice in the three groups received orally the appropriate treatment for 15 days. Post treatment, two criteria were chosen as indicators for the efficacy of the extract. The first one was relative testicular weight to body weight. The other one was the concentration of sperm in caudal part of epidydemis. The results of this experiment revealed that there was a significant (P<0.01) increase in relative testicular weight/body weight in group that was treated with 125mg/kg/day compared with control group. However, the second group which was treated with 200 mg/kg B.W showed significant decrease in testis weight /body weight as compared to control group and first group. From the present study, one can conclude that the alcoholic extract of Cocos nucifera fruit does not toxic to the laboratory mice and the low dose (125mg/kg B.W.) causes enhancement in male fertility of mice, while higher dose (200mg/kg B.W.) causes reduction in sperm concentration and relative testicular weight/body weight compared with the first dose.