FORMATION OF SOMATIC EMBRYOS FROM FLOWER BUDS OF DATE PALM

Abstract

Results showed that the female flowers of date palms cultured in vitro were able to form callus from its meristimatic cells. Flowers which were taken at first(influences are complete but still not seen in the axial of leaves) and second stage (influences started to release out of leave axial) were superior in callus formation and quality of callus compared with that taken at third stage( Complete influences and grown out of leave axial but not opened and ready for pollination) . Results also showed no significant differences between 50 and 100mg/L of 2,4-D in their effect on percentage of callus induction and quality of callus producted. Whereas, NAA at 30mg/L has more significant effect than 15mg/L on callus induction and quality of callus producted. Primary callus which were produced from first and second stages were developed to nodular callus which was a primordia of somatic and cylinderical embryos.