Effect of exposure time of laser He-Ne 632.8 nm,1 m/watt on wound healing process in rabbits


The objective of this work was to assess a compare grossly and microscopically, the effect of exposure time of He-Ne 632.8 nm. 1 m/watt in wound healing in rabbits. For this purpose 15 adult male rabbits were used, divided into 3 groups, 5rabbits in each group. Xylazine 20 mg/kg and ketamine 10mg/kg used I/m to induce general anesthesia. Surgical open wound was made and immediately. The 1st,2nd and 3rd groups were exposure to 632.8 nm. 1 m/watts low level laser during 3,5 and 7minte. All the groups exposure was done on 1st, 3rd , 5th and 7th day. The spacement for histological studies taken on 3rd, 5th and 7th days and immediately kept in 10% buffer formaline for 24 hours. The result revealed that among three groups were the wound closed by fifth day under microscopic examination the slide show complete remodeling and natural skin layers of dermis, epidermis in comparison with groups first and 3rd. The 1st group shows fibroblast and collagen fibers fill the gap, while the third group shows mature normal structure at same stage.