Antimicrobial Activity of Lawsonia inermis L. Extracts

Abstract

Antibacterial activity was tested for three Hena ( Lawsonia inermis L.) extracts , These extracts including hot ethanolic 70% extract , flavonoid extract and oil extract , they were tested against five bacterial species including Escherechia coli , Staphylococcus aureus and Pseudomonas aeruginosa , Streptococcus spp, Klebsella pneumoniae . And The antifungal activity evaluated against (Candida albicans, Aspergillus niger ) .The results showed that flavonoid extract exhibited best antimicrobial activity followed by hot ethanolic 70% extract, while oil extract showed no antimicrobial activity .In addition the flavonoid extract has been separated to precipitate and supernatant and study its antibacterial activity against (E.coli, S.aureus). The precipitate portion exhibited highest activity(38mm) against S.aureus and (32mm)against E.coli.The flavonoid components extract was examined by thin layer chromatography which showed that the flavonid extract was composed of four components, these components were separated quantity by using column chromatography, each separated component was tested for antibacterial activity against (E.coli, S.aureus).The component (III) revealed moderate antibacterial activity(17mm)against ( S.aureus), (14mm) against(E.coli )and the component(IIII) revealed (8,7mm) respectively , while components(I, II) showed no antibacterial activity .