Co-Expression of Intestinal Lymphocyte Subpopulations in Ulcerative Colitis: An Analysis by Dual- Immunofluorescence Technique

Abstract

background: Fluoresceins labelled monoclonal antibodies against cell surfaceantigens are able directly to examine the expression of multiple antigens on thesurface of lymphoid cells in Ulcerative colitis lesions.Materials and methods: Using dual-immunofluorescence for investigated thecoexpression of (CD3-CD19 and CD4-CD8) antigens on infiltrated lymphocytes of 47patients with ulcerative colitis and 10 subjects from control group. Total anddifferential T cells as well as plasma cell counts were recorded in all tissue samples.Non parametric Kruskal-Wallis test was used to compare the median cell countsamong different study groups.Results: T lymphocyte, T-cell subtype and plasma cell in UC were high with highlystatistical significant difference when compared with those in normal control subjectsT cell UC (301.39) Controls (35.48), T-helper 2 UC (228.41) Controls (24.75), Thelper1 UC (54.21) Controls (13.45) and plasma cell UC (45.46) Controls (12.94).Recorded values of were different and increased according to modified Geboeshistological grades of disease activity T lymphocytes (r=0.944) followed by T helper2 subtype (r=0.821), T helper 1 (r=0.653) and B lymphocyte (r=0.237).Conclusions: The increased number of lymphocyte subsets was found in the UClesions and correlated with different histological grades of disease activity.